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Luciferase is a general term for enzymes that can produce bioluminescence in nature, with the most representative ones are F-Luciferase from fireflies and R-Luciferase from renilla. Under the catalysis of luciferase, luciferin can efficiently convert to oxidized luciferin and emit strong fluorescence at the same time. Therefore, researchers used luciferase to develop a highly sensitive and convenient gene reporter system. Currently, this system is widely applied in various fields, including transcription factor binding site and promoter activity analysis, RNA degradation, signal transduction, drug screening, and in vivo animal imaging.